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RT-PCR Primer Design failed to search for exon annotations (Read 78 times)
Nov 14th, 2017 at 10:03pm

fbalao   Offline
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Hi,
I'm having a error using the RT-PCR Primer Design. It seems UGENE can't detect my exon annotations. Any idea?


Task report [Search primers to annotations]
Error: Subtask {FindExonRegionsTask} is failed: Failed to search for exon annotations. The sequence scaffold1126 doesn't have any related annotations.

Thanks in advance
 

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Reply #1 - Nov 15th, 2017 at 10:01am

Olga Golosova   Offline
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Hi!
I'm not able to reproduce the issue using our sample data. Could you please send us the scaffold1126 sequence (e.g. by email)?
 
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Reply #2 - Nov 15th, 2017 at 3:25pm

fbalao   Offline
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Yes sure. Here, the scaffold and the annotation file. I'm using UGENE V1.26.3 in Ubuntu 14.04.
 
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Reply #3 - Nov 16th, 2017 at 6:46pm

Olga Golosova   Offline
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It appears, that UGENE incorrectly processes annotations in case they are stored in a separate document. So as a workaround, you need to export the sequence and the annotations into a single GenBank file. See the screenshots and the result file in the attachment.

Thanks for informing us about this problem!
 
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