Plasmid Auto Annotation

The Plasmid Auto Annotation feature allows you to automatically annotate possible functional elements of a given sequence, such as promoters, terminators, origins of replication, known genes, common primers, and other features. Conceptually, this functionality is similar to that offered by the PlasMapper software. The database for plasmid auto-annotation is based on the following resource.

To activate Plasmid Auto Annotation on your sequence, use the menu item Analyze ‣ Annotate plasmid. In the dialog that appears, you can select the features to search in the sequence.

The detected plasmid features are stored as automatic annotations and can be controlled through the corresponding menu. Refer to Automatic Annotations Highlighting to learn more.

Plasmid Auto Annotations consist of 471 carefully selected and annotated plasmid features broken down into 12 feature categories. These are shown in the following table:

This feature database has been created by combining the original FASTA formatted PlasMapper 2.0 feature database (which had 336 features) with AddGene’s sequence feature database, followed by extensive manual editing, duplicate removal, validation, and cleaning.