Molecular Cloning in silico

This chapter describes a set of tools in UGENE to perform molecular cloning experiments in silico.

This allows you to digest a molecule into fragments, create a fragment from a sequence region, and ligate fragments into a new molecule.

• Digesting into Fragments
• Creating Fragment
• Constructing Molecule
  • Available Fragments
  • Fragments of the New Molecule
  • Changing Fragments Order in the New Molecule
  • Removing Fragment from the New Molecule
  • Editing Fragment Overhangs
  • Adjusting Fragment Ends
  • Reverse Complement a Fragment
  • Other Construction Options
  • Output
• Creating PCR Product