Creating Fragment

To create a DNA fragment from a sequence region, activate the Sequence View window and select either the Actions ‣ Cloning ‣ Create Fragment item in the main menu or the Cloning ‣ Create Fragment item in the context menu.

The Create DNA Fragment dialog appears:

If a region has been selected, you can choose to create the fragment from this region. Otherwise, you can either choose to create the fragment from the whole sequence or select the Custom item and input the custom region.

To add a 5’ overhang to the direct strand, check the Include Left Overhang checkbox and input the required nucleotides. To add a 5’ overhang to the reverse strand, in addition to the described steps, select the Reverse-complement item in the same group box.

Similarly, to add a 3’ overhang, check the Include Right Overhang checkbox, input the required overhang, and select either the direct or the reverse-complement strand.

On the Output tab of the dialog, you can optionally modify the annotations output settings.

Finally, press the OK button to create the fragment. The fragment will be saved as an annotation.