Creating PCR Product

To create a PCR product from a primer, use the Cloning->Create PCR product context menu of the primer annotation.

The Create PCR Product dialog appears:

If a primer has been selected, you can choose to create the PCR product from this primer. Otherwise, you can either choose to create the PCR product from the whole sequence or select the Custom item and input the custom region.

To add a 5’ overhang to the direct strand, check the Include Left Overhang checkbox and input the required nucleotides. To add a 5’ overhang to the reverse strand, in addition to the described steps, select the Reverse-complement item in the same group box.

Similarly, to add a 3’ overhang, check the Include Right Overhang checkbox, input the required overhang, and select either the direct or the reverse-complement strand.

On the Output tab of the dialog, you can optionally modify the annotations output settings.

Finally, press the OK button to create the PCR product. The PCR product will be saved as an annotation.