Sanger Reads Consensus
Each base of a consensus sequence is calculated as a function of the corresponding column bases. The Sanger Reads Editor allows switching between different consensus modes: Simple extended and Strict.
The Simple extended algorithm selects the best character from the extended DNA alphabet. Only bases with frequencies greater than a threshold value are taken into account.
The Strict algorithm returns a gap character (’-’) if the symbol frequency in a column is lower than the specified threshold.
To switch the consensus mode, go to the Consensus tab of the Options Panel:
