Chromatogram Viewer

The Chromatogram Viewer plugin adds DNA chromatogram data viewing and editing capabilities to UGENE.

Currently, the supported chromatogram file formats are ABIF and SCF.

To view a chromatogram, simply open a relevant file in UGENE using standard methods (e.g., drag & drop the file or press the Ctrl-O shortcut). The Chromatogram Viewer is automatically embedded into the generic Sequence View if chromatogram data are detected, as shown in the screenshot below:

After zooming in, more chromatogram details are available:

To edit sequence data, right-click on the chromatogram view and select the Edit new sequence option in the context menu that appears. The following dialog will appear:

Select the new document format and location and click on the Create button.

The original DNA sequence cannot be changed; however, you can add and modify a new sequence stored in a separate file.

The sequence being edited is displayed right above the original one. Symbols can be changed by clicking on an interesting value, and modifications are shown in bold.

Additionally, you can show or hide different signals of the chromatogram and quality bars with the help of the Show/hide trace and Show quality bars toolbar buttons correspondingly:

• Exporting Chromatogram Data
• Viewing Two Chromatograms Simultaneously