Remote BLAST Element

Finds annotations for the supplied DNA sequence in the NCBI remote database.

Element type: blast

Parameters

ParameterDescriptionDefault valueParameter in Workflow FileType
DatabaseSelects the database to search through. Available databases are blastn, blastp, and cdd.ncbi-blastndbstring
Available values are:
* ncbi-blastn
* ncbi-blastp
* ncbi-cdd
Expected valueThis parameter specifies the statistical significance threshold of reporting matches against the database sequences.10e-valstring
Results limitThe maximum number of results.10hitsnumeric
MegablastUse megablast.Falsemegablastboolean
Short sequenceOptimizes search for short sequences.Falseshort-sequenceboolean
Entrez queryEnter an Entrez query to limit search.entrez-querystring
Annotate asName of the result annotations.result-namestring
BLAST outputLocation of the BLAST output file. This parameter is insignificant for cdd search.blast-outputstring
Gap costsCost to create and extend a gap in an alignment.2 2gap-costsstring
Match scoresReward and penalty for matching and mismatching bases.1 -3match-scoresstring

Input/Output Ports

The element has 1 input port:

Name in GUI: Input sequence

Name in Workflow File: in-sequence

Slots:

Slot In GUISlot in Workflow FileType
Sequencesequencesequence

And 1 output port:

Name in GUI: Annotations

Name in Workflow File: out-annotations

Slots:

Slot In GUISlot in Workflow FileType
Set of annotationsannotationsannotation-table