Extract Coverage from Assembly

This workflow sample extracts coverage and/or base counts from input assemblies. It supports multiple input assemblies and produces a tab-delimited plain text output for each of them. Coverage values are filtered based on the provided threshold parameter.


How to Use This Sample

If you haven’t used workflow samples in UGENE before, refer to the How to Use Sample Workflows section of the documentation.


Workflow Sample Location

The workflow sample “Extract Coverage from Assembly” is available in the “NGS” section of the Workflow Designer samples.


Workflow Image

The workflow appears as follows:

Workflow Image


Workflow Wizard

The wizard consists of 3 pages:


1. Input Assembly(ies)

On this page, input one or more assembly files.

Input Page


2. Extract Parameters

Configure the extraction parameters:

Extract Parameters Page

ParameterDescription
FormatOutput file format (tab-delimited plain text).
ExportType of data to export: coverage or base counts.
ThresholdMinimum coverage value to include in the output.

3. Output Data

On this page, select the destination for the output file.

Output Page


Output Example

Each output file will contain coverage data per base or region, based on the selected export mode and threshold.