From this chapter you can learn how to search for restriction sites on a DNA sequence.
The restriction sites found are stored as automatic annotations. This means that if the automatic annotations highlighting is enabled then the restiction sites are searched and highlighted for each nucleotide sequence opened. Refer Automatic Annotations Highlighting to learn more.
Open a DNA sequence in and click the following button on the Sequence View toolbar:
<center> <br> <img src="/wiki/download/attachments/4227631/Restriction Analysis.png"/> <br> </center>
Alternatively, select either the Actions ‣ Analyze ‣ Find restriction sites item in the main menu or the Analyze ‣ Find restriction sites item in the context menu.
The Find restriction sites dialog appears:
<center> <br> <img src="/wiki/download/attachments/17468941/Restriction Analysis_1.png"/> <br> </center>
You can see the list of restriction enzymes that can be used to search for restriction sites. Also you can set a region to search for. The information about enzymes was obtained from the REBASE database. For each enzyme in the list a brief description is available (the accession ID in the database, the recognition sequence, etc.). If you’re online you can get more detailed information about an enzyme selected by clicking the REBASE Info button.